TABLE 1.
Fission yeast strains used in this study
| Strain | Mating type | Genotype |
|---|---|---|
| LD327 | h− | his3-D1 |
| DY15603 | h+ | his3-D1 mdb1Δ::hphMX leu1-32::P81nmt1-Mdb1-wt-GFP (leu1+) |
| DY15615 | h+ | ura4-D18 his3-D1 leu1-32::Pnmt1-GFP (leu1+) |
| DY15616 | h+ | ura4-D18 his3-D1 leu1-32::Pnmt1-Mdb1-wt-GFP (leu1+) |
| DY15627 | h+ | his3-D1 mdb1Δ::hphMX ars1::CFP-atb2::LEU2 leu1-32::P81nmt1-Mdb1-wt-GFP (leu1+) |
| DY15630 | h+ | his3-D1 mdb1Δ::hphMX leu1-32::P81nmt1-GFP (leu1+) |
| DY15638 | h+ | his3-D1 mdb1Δ::hphMX leu1-32::P81nmt1-Mdb1 (105–624)-GFP (leu1+) |
| DY16307 | h+ | his3-D1 mdb1Δ::hphMX leu1-32::P81nmt1-LZ-Mdb1 (105–624)-GFP (leu1+) |
| DY16309 | h+ | his3-D1 mdb1Δ::hphMX leu1-32::P81nmt1-GST-Mdb1 (105–624)-GFP (leu1+) |
| DY16573 | h− | ura4-D18 his3-D1 (?) crb2 (1–778)F400A mdb1Δ::natMX leu1-32::P81nmt1-GST-Mdb1 (105–624)-GFP (leu1+) |
| DY16579 | h− | ura4-D18 his3-D1 (?) crb2 (1–778)F400A mdb1Δ::natMX leu1-32::P81nmt1-LZ-Mdb1 (105–624)-GFP (leu1+) |
| DY16581 | h− | ura4-D18 his3-D1 (?) crb2 (1–778)F400A mdb1Δ::natMX leu1-32::P81nmt1-GFP (leu1+) |
| DY16639 | h+ | his3-D1 mdb1Δ::hphMX ars1::CFP-atb2::LEU2 leu1-32::P81nmt1-Mdb1 (105–624)-GFP (leu1+) |
| DY16643 | h+ | his3-D1 mdb1Δ::hphMX ars1::CFP-atb2::LEU2 leu1-32::P81nmt1-GST-Mdb1 (105–624)-GFP (leu1+) |
| DY16645 | h+ | his3-D1 mdb1Δ::hphMX ars1::CFP-atb2::LEU2 leu1-32::P81nmt1-LZ-Mdb1 (105–624)-GFP (leu1+) |
| DY17124 | h− | ura4-D18 his3-D1 (?) crb2 (1–778)F400A mdb1Δ::natMX leu1-32::P81nmt1-Mdb1-wt-GFP (leu1+) |
| DY17126 | h− | ura4-D18 his3-D1 (?) crb2 (1–778)F400A mdb1Δ::natMX leu1-32::P81nmt1-Mdb1 (105–624)-GFP (leu1+) |
| DY17216 | h+ | ura4-D18 his3-D1 leu1-32::Pnmt1-Mdb1 (105–624)-GFP (leu1+) |
| DY17218 | h+ | ura4-D18 his3-D1 leu1-32::Pnmt1-GST-Mdb1 (105–624)-GFP (leu1+) |
| DY17220 | h+ | ura4-D18 his3-D1 leu1-32::Pnmt1-LZ-Mdb1 (105–624)-GFP (leu1+) |
| DY18067 | h+ | his3-D1 mdb1Δ::hphMX leu1-32::P81nmt1-GFP-Mdb1-wt (leu1+) ars1::P81nmt1-mCherry-Mdb1-wt (his3+) |
| DY18069 | h+ | his3-D1 mdb1Δ::hphMX leu1-32::P81nmt1-GFP-Mdb1-wt (leu1+) ars1::P81nmt1-mCherry-Mdb1 (105–624) (his3+) |
| DY23263 | h+ | ura4-D18 leu1-32 mdb1Δ::hphMX leu1-32::P81nmt1-Mdb1(105–624)-wt-GFP(leu1+) his3::Ptub1-Mdb1(105–624)-wt-GBP(kanMX) |
| DY24110 | h+ | ura4-D18 leu1-32 mdb1Δ::hphMX leu1-32::P81nmt1-Mdb1(105–624)-wt-GFP(leu1+) his3::Ptub1-Mdb1(105–624)-K434 M-GBP(kanMX) |
| DY24112 | h+ | ura4-D18 leu1-32 mdb1Δ::hphMX leu1-32::P81nmt1-Mdb1(105–624)-K434 M-GFP(leu1+) his3::Ptub1-Mdb1(105–624)-wt-GBP(kanMX) |
| DY24114 | h+ | ura4-D18 leu1-32 mdb1Δ::hphMX leu1-32::P81nmt1-Mdb1(105–624)-K434 M-GFP(leu1+) his3::Ptub1-Mdb1(105–624)-K434 M-GBP(kanMX) |
| DY24566 | h+ | leu1-32 his3-D1 mdb1Δ::hphMX leu1-32::P81nmt1-GFP-GST-Mdb1 (105–624) (leu1+) ars1::P81nmt1-mCherry-Mdb1 (105–624) (his3+) |
| DY24567 | h+ | leu1-32 his3-D1 mdb1Δ::hphMX leu1-32::P81nmt1-GFP-GST-Mdb1 (105–624) (leu1+) ars1::P81nmt1-mCherry-GST-Mdb1 (105–624) (his3+) |
| DY24570 | h+ | leu1-32 his3-D1 mdb1Δ::hphMX leu1-32::P81nmt1-GFP-LZ-Mdb1 (105–624) (leu1+) ars1::P81nmt1-mCherry-Mdb1 (105–624) (his3+) |
| DY24571 | h+ | leu1-32 his3-D1 mdb1Δ::hphMX leu1-32::P81nmt1-GFP-LZ-Mdb1 (105–624) (leu1+) ars1::P81nmt1-mCherry-LZ-Mdb1 (105–624) (his3+) |