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AAG-associated APE1 activity inhibition and AP-DNA binding. A, WT APE1 endonuclease activity on naked AP-Out DNA in the presence of different concentrations of AAG glycosylase. Upper bands are the DNA substrate, lower bands are the endonuclease cleavage product. B, non-denaturing polyacrylamide gel showing binding of AAG to naked AP-Out DNA at different concentrations.
