TABLE 1.

DNA oligonucleotides used in this study

Name	Sequence
Template	5′-ATGGGACGTGCTGTCAGTGCACTGTGACCGTCAGGATGACACGC-3′
Upstream	5′-GCGTGTCATCCTGACGGTCACAGTG-3′
FlapXa
X = 12R	5′-CAGCAGCAGCAGCACTGACAGCACGTCCCAT-3′
X = 14M	5′-CTCGATCAGCTACGCACTGACAGCACGTCCCAT-3′
X = 30M	5′-CGCACTCTCTGTCAGACTCGATCAGCTACGCACTGACAGCACGTCCCAT-3′
X = 30R	5′-CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCACTGACAGCACGTCCCAT-3′
X = 45M	5′-GGTTGTAAATAAGCTCTCACCCTCAGTCAGACTCGATATACAGCGCACTGACAGCACGTCCCAT-3′
X = 45R	5′-CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCACTGACAGCACGTCCCAT-3′
X = 60M	5′-AGATGTCCGTGATTCGGTTGTAAATAAGCTCTCACCCTCAGTCAGACTCGATATACAGCGCACTGACAGCACGTCCCAT-3′
X = 60R	5′-CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCACTGACAGCACGTCCCAT-3′

^a The 5′-flap region is underlined. The length of the flap (nucleotides) in the 5′-single flap species and sequence of the flap (either mixed sequence (M) or CAG repeat (R)) are indicated in the subscript (X).