TABLE 2.

Kinetic parameters of FEN1 acting on flap-containing substrates

Flap length (nt)	kSTOa		kburstb		kcatb
	Mixed sequence	(CAG)n	Mixed sequence	(CAG)n	Mixed sequence	(CAG)n
	s−1		s−1		s−1
12	NDc	54 ± 8	NDc	21 ± 6	NDc	4.4 ± 0.2
14	33 ± 6	NDc	42 ± 10	NDc	2.9 ± 0.1	NDc
30	49 ± 9	36 ± 9	36 ± 8	33 ± 18	2.0 ± 0.1	3.4 ± 0.1
45	12 ± 1	0.95 ± 0.20	6.4 ± 3.0	NAd	1.7 ± 0.1	0.40 ± 0.01
60	3.0 ± 0.7	0.104 ± 0.004	NAd	NAd	1.43 ± 0.04	0.071 ± 0.001

^a Measured at 37 °C under single-turnover conditions.

^b Measured at 37 °C under multiple-turnover conditions.

^c ND, not determined.

^d NA, not applicable because no burst phase was observed.