Fig. 2.

Learning is not affected by overexpression of wild-type N⁺ (hs-N⁺). Flies were subjected to 30 min of heat shock at 37°C followed by 3 h of rest. (a) Learning scores were similar for controls and hs-N⁺ flies regardless of whether they were subjected to heat-shock treatment (n = 2, 4, 2, and 4 for data points from left to right). (b) Heat shock induced expression of hs-N⁺ cDNA in adult heads. Semiquantitative RT-PCR using N primer pairs N1–N2, N3–N4, and N5–N6 shows induction of the hs-N⁺ transgene by 30 min of heat shock at 37°C followed by 3 h of rest (lane C) when compared with PCR from control flies that were kept at 18°C (lane A) or 25°C (lane B). The rp49F-R control primers show no temperature-induced increase in expression of rp49. Details of PCR primer pairs and expected products are given in Materials and Methods. Three separate mRNA isolations showed the same pattern of increased expression of the hs-N⁺ transgene after 37°C heat shock.