Table 1.
List of Bacterial Strains, plasmid and Primers used in the present study.
| Bacterial strains | Description/Purpose | Source/References |
|---|---|---|
| E. coli | ||
| DH5α | Cloning | Invitrogen, USA. |
| BL21 (DE3) | For expression of TlyA | Invitrogen, USA. |
| M. smegmatis | ||
| mc2155 | Wild type | Noens et al., 2011 |
| MB692 | mc2155, ΔTatA | McDonough et al., 2005 |
| JM567 | mc2155, ΔTatC | McDonough et al., 2005 |
| NR116 | mc2155, ΔSecA2 | Gibbons et al., 2007 |
| M. marinum | Wild type | Gao et al., 2004 |
| PLASMIDS | ||
| pET28a+ | For expression in BL21 (DE3) | Novagen, USA. |
| pMyNT | For expression in M. smegmatis mc2155 | Noens et al., 2011 |
| pET28a+ | For expression of GFP | Mittal et al., 2014 |
| PRIMERS | ||
| MtbTlyA | ||
| Forward | 5′-TATATATCCATGGCTCGACGTGCCCGCG-3′ | |
| Reverse | 5′-TATATAGAATTCTTACGGGCCCTCGCTAATCGC-3′ | |
| MsTlyA | ||
| Forward | 5′-TGAGGAGCTAGCGTGGCACGGCGAGC 3′ | |
| Reverse | 5′- TCATAAGCTTTTGCGGCCCTTCC 3′ | |