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. 2015 Aug 21;6:645. doi: 10.3389/fpls.2015.00645

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Copyright © 2015 Parida, Verma, Yadav, Ambawat, Das, Garg and Jain.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of amplification and polymorphic potential of selected SSRs and allelic variations in chickpea genotypes. (A) Polymerase chain reaction based validation of amplification and polymorphic potential of selected SSRs in chickpea genotypes. Only five selected examples of SSRs have been represented. Representative gels showing PCR amplification of polymorphic SSRs (labeled on right side) validating the length polymorphism between desi and kabuli chickpea genotypes (genotype details are available in Supplementary Table S3). M, 50 bp DNA ladder as size standard. (B) Eight different representative allele types identified based on the fragment length polymorphism pattern of 160 SSR markers across desi and kabuli chickpea genotypes. The SSR marker-alleles are illustrated according to their lower to higher fragment size (bp).