FIGURE 7.

Effect of IFN-γ, TNF-α, or perforin deficiency on IL-15 SA–mediated immunotoxicity. IFN-γ KO, TNF-α KO, Pfn KO mice, and WT controls received 2 μg IL-15 SA treatment for 4 d. (A–C) Body temperature was measured at 24 h after the last IL-15 SA treatment. Untreated WT mice were included as control. Splenic NK numbers (D) and CD69 expression (E and F) were measured using flow cytometry. n = 9–16 mice/group. Data are representative of two to three separate experiments. *p < 0.05 compared with untreated WT control, ⁺p < 0.05 compared with IL-15 SA–treated WT mice.