Figure 1. Functional effect of RNA and ssDNA on the SERCA/PLN complex.

(a) Dissociation constants as measured by both fluorescence polarization and affinity capillary electrophoresis of both RNA and ssDNA. (b) pK_Ca of SERCA as measured by coupled enzyme assays. Dissociation constants versus ssDNA length obtained from fluorescence polarization (c) and affinity capillary electrophoresis (d). Sequences for (c) and (d) are found in Table 1.