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. 2015 Aug 21;5:13390. doi: 10.1038/srep13390

Figure 2. Validation of the mRNA and protein changes of the selected genes.

Figure 2

Nine genes in the “Superpathway of Cholesterol Biosynthesis” (A) and thirteen genes in the “Axonal Guidance Signaling” (B) were randomly selected and their mRNA levels were determined by qRT-PCR in PANC-1 cells untreated or treated with the combination of metformin (5 mM) and Aspirin (2 mM) for 48 h. The protein levels of the selected genes RET, CCL2, and ANGPTL4 were determined by Western blotting (C). PANC-1 cells were treated with metformin (5 mM) and aspirin (2 mM), alone or in combination for 24, 48 and 72 h, respectively. β-actin was used as a loading control. Cont, control; Met, metformin; Asp, aspirin; Comb, the combination of metformin and aspirin.