Table 2. Summary of CRISPR/Cas9-induced site-specific insertion with ssODN through HDR.

Injected components	No. of tested blastocysts	HDR blastocysts (% of tested)	Precise HDR (% of tested)
Cas9 mRNA + R1 sgRNA + ssODN (80 ng/μL)	23	3 (13.04)	2 (8.70)
Cas9 mRNA + R1 sgRNA + ssODN (10 ng/μL)	20	0	0
Cas9 mRNA + F2 sgRNA + ssODN (80 ng/μL)	19	0	0

Different pools as indicated were coinjected into mature MII pig oocytes. After parthenogenetic activation, oocytes were cultured to the blastocyst stage and a single blastocyst was selected for genotyping by RFLP and Sanger sequencing assays.