Figure 3. T cell proliferation and activation in Padi4 KO mice after the GPI immunization.

(A–C) The numbers of CD3⁺ T cells, CD4⁺ CD62L⁻ CD44⁺ memory T cells, and CD4⁺ CD62L⁻ CD69⁺ activated T cells in spleens were counted 0, 7, and 14 days after the immunization. Pre-immunized (WT n = 3, Padi4 KO n = 3), 7 days after the immunization (WT n = 6, Padi4 KO n = 6), 14 days after the immunization (WT n = 11, Padi4 KO n = 13). (D) The number of CD4⁺ CD25⁺ Foxp3⁺ regulatory T cells in spleens was counted 14 days after the immunization (WT n = 8, Padi4 KO n = 10). Gray bars; WT, open bars; Padi4 KO.