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A partial transfection assay validates EGFR-directed nanoparticle uptake of EGF-HPPS. Cellular uptake of (DiR-BOA)HPPS, EGF(DiR-BOA)HPPS, EGF(DiR-BOA)HPPS with HDL or EGF(DiR-BOA)HPPS with excess of HDL, and EGF by EGFR-GFP-LDLA7 cells. White arrow represents specific uptake of EGF(DiR-BOA)HPPS through EGFR-mediated pathway. Blue arrow indicates the cells without EGFR-GFP expression have no uptake of EGF(DiR-BOA)HPPS
