Figure 5.

(A) Immunoblot detection of VEGFR2 protein expression in retinal arterioles from three pigs. (B) Immunofluorescence detection of VEGFR2 in an isolated porcine retinal arteriole. In the presence of anti-VEGFR2 (green) or anti-eNOS (red) antibodies, high levels of immunostaining were detected in the endothelium for VEGFR2 and eNOS, and in the smooth muscle layer for VEGFR2. The merged image shows overlap staining (orange-yellow) in the endothelial layer. White arrowheads denote endothelial cells and white arrow denotes smooth muscle cells. Data shown are representative of three separate experiments.