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. 2014 Feb 5;141(6):561–575. doi: 10.1007/s00418-014-1184-3

Table 2.

Fluorophores used in live-cell single-molecule localization microscopy studies

Fluorophore λabs/λem (nm) Photons/on-event Tag Additives Camera frame rate (Hz) Temporal resolution (s) Spatial resolution References
Oregon green Rhodamine 490/514 900 Snap None 100–150 30 3D: 50 nm xy; 90 nm z Jones et al. (2011)
Alexa 488 Rhodamine 496/520 400 Snap Glutathione, oxygen scavenger 30 150 n/a Benke et al. (2012)
PA-GFPa FP 504/517 n/a fusion protein None 5–10 100–300 40 nm Hess et al. (2007)
PA-GFPa FP 504/517 n/a fusion protein None 30 90 25 nm Wilmes et al. (2012)
505 Rhodamine 504/532 300–2,000 Snap None 30–100 60–300 n/a Klein et al. (2011)
EYFP FP 514/527 741 fusion protein None 10 60–120  40 nm Biteen et al. (2008)
Dil Carbocyanine 549/565 720 none Oxygen scavenger 500 15  40 nm Shim et al. (2012)
TMR Rhodamine 554/580 1,100 Snap None 100–150 30  3D: 45 nm xy; 82 nm z Jones et al. (2011)
TMR Rhodamine 554/580 300–2,000 Snap None 30–100 60–300 n/a Klein et al. (2011)
TMR Rhodamine 554/580 n/a Clip Gluthatione, oxygen scavenger 30–50 200–350  n/a Klein et al. (2012)
TMR rhodamine 554/580 n/a Halo None 31 6–16  15 nm Appelhans et al. (2012)
PATagRFPa FP 562/595 n/a fusion protein None 30 90  25 nm Wilmes et al. (2012)
tdEosa FP 569/581 750 fusion protein None 25  25–60  60 nm Shroff et al. (2008)
tdEosa FP 569/581 1,200 fusion protein None 100–150  3D: 30  3D: 40 nm xy; 80 nm z Jones et al. (2011)
EosFPa FP 571/581 n/a fusion protein None 20  500  25 nm Manley et al. (2008)
mEos2a FP 573/584 1,200 fusion protein None 100–150  3D: 30 3D: 40 nm xy; 80 nm z Jones et al. (2011)
Alexa 568 Rhodamine 572/600 1,700 directly labeled β-Mercaptoethanol, oxygen scavenger 500  3D: 30 3D: 40 nm xy; 70 nm z Jones et al. (2011)
LysoTracker Red Bodipy 577/590 820 none Oxygen scavenger 500  1  30 nm Shim et al. (2012)
MitoTracker Red Cationic rosamine 581/644 790 none Oxygen scavenger 500–900  2–10  30–40 nm Shim et al. (2012)
ER-Tracker Red Bodipy 587/615 820 none Oxygen scavenger 500  10  35 nm Shim et al. (2012)
Alexa 647 cyanine 649/670 3,500 directly labeled, Snap β-Mercaptoethanol, oxygen scavenger 500 

2D: 0.5 

3D: 1–2 

2D: 25 nm

3D: 30 nm xy; 50 nm z

Jones et al. (2011)
Alexa 647 Cyanine 649/670 n/a Snap Gluthatione, oxygen scavenger 30–50  200–350 n/a Klein et al. (2012)
SiR Silicon-rhodamine 650/668 630 Snap None 50  200  n/a Lukinavicius et al. (2013)
ATTO 655 Oxazine 663/684 500–1,500 TMP None 50  10  20 nm Wombacher et al. (2010)
ATTO 655 Oxazine 663/684 1,200 Snap None 100–150  30  3D: 40 nm xy; 80 nm z Jones et al. (2011)
ATTO 655 Oxazine 663/684 800 Halo None 30  90  25 nm Wilmes et al. (2012)

FP fluorescent protein

aconverted/activated form