Skip to main content
. Author manuscript; available in PMC: 2016 Oct 15.
Published in final edited form as: Physiol Behav. 2015 Feb 19;150:2–7. doi: 10.1016/j.physbeh.2015.02.027

Figure 1.

Figure 1

For each experiment, animals were injected into the PVN at time 0, then placed immediately into restrainers. Blood samples were obtained by tail clips immediately upon restraint, and at 15 and 30 minutes after the injections. Animals remained in restraint through the 30 minute time point. For the AM-251 experiment, a fourth blood sample was taken at 60 minutes, and animals were perfused 120 minutes after injection. For the other two experiments, animals were sacrificed by rapid decapitation at the 60 minute time point, and trunk blood obtained for the 60 minute time point.