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Chondrogenic differentiation of human bone marrow-derived MSCs in collagen sponges after 28 days of culture in presence of TGF-β1 versus defined chondrogenic ITS medium. TGF-β1-induced matrix synthesis inside the sponge (HES: Hematoxylin-Eosine-Saffron) rich in proteoglycans (Alcian blue) and collagens (Sirius Red), particularly in type-II collagen.
