Table 1.
Effects of IFP-conditioned media on gene expression in patient-matched chondrocytes and synoviocytes
| Genes | Chondrocytes | Synoviocytes |
|---|---|---|
| iNOS | 427.22 ± 123.76* | 2.63 ± 0.45* |
| COX-2 | 57.39 ± 12.85* | 362.75 ± 145* |
| mPGES-1 | 41.25 ± 7.51* | 13.64 ± 1.82* |
| MMP13 | 18.29 ± 2.96* | ND |
| ADAMTS-4 | 86.64 ± 21.13* | ND |
| IGF-I | 8.86 ± 2.04* | 4.60 ± 2.64 |
| TGF-β1 | 1.49 ± 0.28 | 1.09 ± 0.16 |
| Type-II collagen | 1.43 ± 0.19 | ND |
| Aggrecan | 1.27 ± 0.18 | ND |
The expression of inflammatory genes (iNOS, mPGES-1, COX-2) and of genes coding for matrix components (type-II collagen, aggrecan) or for factors involved in cartilage remodelling (MMP13, ADAMTS-4, IGF-I, TGF-β1) was analyzed in patient-matched cells (n = 20) by real-time quantitative PCR after 24 h of culture in paired conditioned medium. Data are expressed as means ± SEM of the mean over control values. *P ≤ 0.05 between IFP conditioned media-treated and control cells.