Fig. 1.

Full-length genomic DNA sequences of DcPSY1 and DcPSY2. a Genomic DNA amplification of DcPSY1 (about 1,972 bp) and DcPSY2 (about 4,015/4,024 bp) from the roots of Ws, Af, E02032, E36101 and E2003. b The genomic DNA sequence of DcPSY1 from ATG to TAG is 1,813 bp and contains 5 exons and 4 introns. The SNP C₆₆G was found in the first exon between Ws and Af, which corresponds to a L₂₂F amino acid change. DcPSY1 of E2003 is from Ws and that of E02032 and E36101 are from Af. c The genomic DNA sequence of DcPSY2 from ATG to TAG is 3,781 and 3,790 bp in Ws and Af, respectively, and contains six exons and five introns. A nine-nucleotide sequence (CGCACCAAC) was found in the third intron between Ws and Af that acts as an InDel marker to differentiate the DcPSY2 of E36101 and E2003 from that of Ws and that of E02032 from that of Af