Fig. 2.

Promoter analysis of PSY genes. a Phylogenetic tree of PSY promoter regions. The promoters of SlPSY1 and SlPSY2 were obtained from the SOL Genomics Network (http://solgenomics.net) and that of AtPSY (At5g17230) was obtained from the P1 clone MKP11. ZmPSY1 (FJ971252.1), ZmPSY2 (AY325302.1), ZmPSY3 (DQ372936.1), OsPSY1 (297606578), OsPSY2 (297613623) and OsPSY3 (297610002) were obtained from the NCBI database. The phylogenetic tree was generated by MEGA 5.05 using the neighbor-joining method (Tamura et al. 2011). b Major putative cis-acting elements in the 5′-untranslated regions of SlPSY1, SlPSY2, OsPSY3, DcPSY1 and DcPSY2. Elements were identified using PlantCare and are shown by different character symbols. Only putative cis-acting elements associated with defense, hormones, circadian regulation and other particular response elements are shown. Motifs positions are relative and not drawn to scale