(A) HPLC analysis identified a specific increase in striatal DA concentration (ng/mg) in the 14-3-3 FKO (N = 7) mice compared with that of WT (N = 5). (B) In the cortex, no difference in monoamine or metabolite content was detected between WT (N = 4) and 14-3-3 FKO mice (N = 7). (C and D) In 14-3-3 FKO mice, transgene expression (green) does not colocalize with the TH-containing (red) DA neurons of the Substantia Nigra pars compacta (SNc) and Ventral Tegmental Area (VTA) (C), nor their projections to the striatum (D). Scale bar, 100 μm (C) or 50 μm (D). Data are presented as mean ± S.E.M., with statistical significance denoted by *p < 0.05, two-tailed t-test.