. Author manuscript; available in PMC: 2016 Sep 1.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Jul 17;1000:105–111. doi: 10.1016/j.jchromb.2015.07.018

Table 1.

Assay accuracy and precision (%CV) for ManNAc and Neu5Ac in LQC, MQC, and HQC samples.

ManNAc	LQC (30.0 ng/mL)	MQC (251 ng/mL)	HQC (4051 ng/mL)
Mean	31.6	243	3922
Accuracy (%)	105.2	96.7	96.8
Intra-assay precision (%CV)	<6.7	<6.5	<4.5
Inter-assay precision (%CV)	4.5	5.1	2.1
Number of replicates (n)	18	18	18

Neu5Ac	LQC (75.0 ng/mL)	MQC (359 ng/mL)	HQC (8159 ng/mL)
Mean	74.6	326	7326
Accuracy (%)	99.4	92.4	91.7
Intra-assay precision (%CV)	<7.6	<10.8	<3.2
Inter-assay precision (%CV)	4.9	3.7	2
Number of replicates (n)	18	18	18