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. Author manuscript; available in PMC: 2015 Aug 21.
Published in final edited form as: Methods Mol Biol. 2015;1332:161–176. doi: 10.1007/978-1-4939-2917-7_12

Figure 1. Flt-1/VEGFR-1 targeting strategy.

Figure 1

A Flt-1/VEGFR-1 targeting vector containing the lacZ (blue box) and neo (green box) genes was used to replace a small fragment (gray box) containing the spcs (signal peptide coding sequence) and the sds (splicing donor site). The targeting vector contained a 6.5 kb 5’ homology arm and 1.6 kb 3’ homology arm. Purple box, 5’ untranslated region; red box, polyadenylation signal (pA); yellow box, thymidine kinase negative selection gene (TK). The resulting targeted allele is shown at the bottom.