FIG. 2.

Effects of CB CD34⁺ differentiation on miR-124 and Tip110 expression. Human core blood CD34⁺ cells were isolated and cultured in the presence of 10% fetal bovine serum and cytokines, 100 ng/mL SCF, 100 ng/mL FLT3 ligand, and 100 ng/mL thrombopoietin, for indicated days. The cells were collected. Separate aliquots were used for staining and flow cytometry analysis for CD34⁺ (A), CD34⁺CD38⁻ (B), and CD34⁺CD38⁻CD45RA⁻CD90⁺CD49f⁺ (C) or RNA isolation and qRT-PCR for Tip110 mRNA level (D, E) and miR-124 level (F, G). β-Actin was included as a reference for Tip110 qRT-PCR (D), and U6 was included as a reference for miR-124 qRT-PCR (E). y-Axis in (A–C) represented the number of cells of the 1 million cells counted. The data are mean±SEM and representative of three independent experiments.