Table 1.
Strains and plasmids used in this work
| Strain/Plasmid | Genotypea | Reference |
|---|---|---|
| P. syringae pv. tomato | ||
| DC3000 | Rifr | [14] |
| ∆iaaL | Kmr | This work |
| ∆matE | Kmr | This work |
| ∆hrpL | Kmr | This work |
| E. coli | ||
| E. coli DH5α | F-, ϕ80dlacZ M15, (lacZYA-argF) U169, deoR, recA1, endA, | [66] |
| hsdR17 (rk − mk −), phoA, supE44, thi-1, gyrA96, relA1. | ||
| Plasmids | ||
| pGEM-T Easy Vector | Ampr, ori f1, lacZ | Promega, USA |
| pGEM-T Vector | Ampr, ori f1, lacZ | Promega, USA |
| pGEM-T-KmFRT-BamHI | Contains Kmr from pKD4 (Ampr Kmr) | [67] |
| pMP220 | Expression Vector, Tcr | [35] |
| pJB3Tc20 | Expression Vector, Tcr | [68] |
| pIAC1 | pGEM-T derivative, contains approx. 0.5 kb on each side of the iaaL gene (Ampr) | This work |
| pIAC2 | pGEM-T derivative, contains approx. 0.5 kb on each side of the hrpL gene (Ampr) | This work |
| pIAC3 | pGEM-T derivative, contains approx. 0.5 kb on each side of the mat gene (Ampr) | This work |
| pIAC1-Km | pGEM-T derivative, contains approx. 0.5 kb on each side of the iaaL gene interrupted by the kanamycin resistance gene nptII (Amp r, Km r) | This work |
| pIAC2-Km | pGEM-T derivative, contains approx. 0.5 kb on each side of the hrpL gene interrupted by the kanamycin resistance gene nptII (Ampr, Kmr) | This work |
| pIAC3-Km | pGEM-T derivative, contains approx. 0.5 kb on each side of the matE gene interrupted by the kanamycin resistance gene nptII (Ampr, Kmr) | This work |
| pMP220-PmatE | pMP220 derivative, contains the lacZ gene expressed from the PmatE promoter (Tcr) | This work |
| pMP220-R2 | pMP220 derivative, contains the lacZ gene expressed from the PiaaL promoter (Tcr) | This work |
| pJB3-matE | pJB3 derivative, contains the matE gene with RBS | This work |
| pJB3-iaaL | pJB3 derivative, contains the iaaL gene with RBS | This work |
aAmpr, Kmr, Rifr and Tcr indicate resistance to ampicillin, kanamycin, rifampicin and tetracycline, respectively