Acquiring image of plates
1.	Turn on machine
2.	Open “Immuno Capture” Version 6.3 software.
3.	Step 1: select plate type.
4.	Step 2: load plate.
5.	Step 3: select scanning options.
6.	Step 4: start scanning.
7.	Obtain overview image of plate.
8.	Eject plate.
9.	Quit “Immuno Capture” software.
Counting the spot forming units
1.	Open “Immuno Spot Capture” Version 5.0 software.
2.	Select object type: normal.
3.	Select counting module: smart count.
4.	Step 1: load plate.
5.	Step 2: define counting parameters: test accuracy of spot recognition on wells with different spots.
6.	Start auto count.
Quality control
1.	Open “Immunospot Capture” Version 5.0 software.
2.	Select counting module: quality control.
3.	Step 1: load plate.
4.	Step 2: Analyze highlighted wells individually.
5.	Finish quality control.