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Arterial protein expression levels of MnSOD and p66Shc, but not Nox2 or 3-NT, were significantly decreased in T2D rats compared to control rats (n = 3 each; P = 0.039 and P = 0.028, resp.; t-test). Representative immunoblots are shown below the summary data. Relative protein levels were calculated as the ratio of the intensity of the chemiluminescent bands to the intensity of the total protein loaded on the gel and are expressed as a percentage of the values measured in control rats.
