Reagents from Compartment Protein Extraction Kit	Volume for 100 mg of tissue	Composition (based on Millipore datasheet cat#21451)
Buffer C	500 μl	HEPES (pH 7.93), MgCl2, KCl, EDT4, Sucrose, Glycerol, Sodium Orthovanadate5
Buffer W	400 μl	HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium Orthovanadate
Buffer N	150 μl x 2	HEPES (pH 7.9), MgCl2, NaCl, EDTA, Glycerol, Sodium Orthovanadate
Buffer W	400 μl	HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium Orthovanadate
Buffer M	100 μl	HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate (DOC)6, NP-406, Sodium Orthovanadate
Buffer CS	200 μl	PIPES (pH 6.8), MgCl2, NaCl, EDTA, Sucrose, Sodium Dodecyl Sulfate (SDS)7, Sodium Orthovanadate
Buffer C	150 μl	HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium Orthovanadate
1x PBS	500 μl/wash	-