| Reagents | Preparation | Final Concentration / Amount for 1 mm-thick pellet (~5–10 mg dry weight) | Volume for 1 mm-thick pellet (~5–10 mg dry weight) |
| Ammonium bicarbonate (NH4HCO3) | 100 mM solution in HPLC-grade water | - | - |
| Urea | 8 M solution in 100 mM ammonium bicarbonate | 8 M | 50 μl |
| Dithiothreitol | Reconstitute in HPLC-grade water at 500 mM | 10 mM | 1 μl |
| Iodoacetamide | Reconstitute in HPLC-grade water at 500 mM | 25 mM | 2.5 μl |
| Peptide-N-Glycosidase F (PNGaseF) | Commercial solution at 500 U/μl | 1,000 U | 2 μl |
| Endoproteinase LysC, mass spectrometry-grade | Reconstitute in HPLC-grade water at 0.5 μg/μl | 1 μg | 2 μl |
| Trypsin, mass spectrometry-grade (round 1) | Commercial solution at 0.5 μg/μl | 3 μg | 6 μl |
| Trypsin, mass spectrometry-grade (round 2) | Commercial solution at 0.5 μg/μl | 1.5 μg | 3 μl |
| Trifluoro-acetic acid (TFA) | 50% solution in HPLC-grade water | - | 2–5 μl |
| Acetonitrile (elution) | 60% solution with 0.1% TFA in HPLC-grade water | - | 500 μl |
| Acetonitrile (reconstitution) | 3% solution with 0.1% TFA in HPLC-grade water | - | 100 μl |
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