Figure 6.

ST production by A. nidulans WT and Δppo mutants after inoculation onto lox3–4 and WT kernels at 3.5 and seven dpi. ST was separated from crude extracts by thin layer chromatography and quantified by densitometry. (A) Effect of maize ZmLOX3 deletion: sections from thin layer chromatograms, demonstrating differences in ST production by A. nidulans strains grown on WT or lox3–4 mutant kernels at seven dpi. The numbers on the right denote ratios (lox3–4:WT) of ST production in infected kernels as measured by densitometry. Values shown are the means of four replicates ± one standard deviation. (B) Effect of A. nidulans Δppo deletions: ST production at three and seven dpi by WT A. nidulans and Δppo mutants grown on maize WT and lox3–4 kernels. Numbers above bars denote the ratios of ST production (Δppo mutant:WT) of A. nidulans within each maize genotype. Values shown are the means of four replicates of five kernels each. Asterisks above bars represent significant differences in ST production for each fungal genotype between WT and lox3–4 mutant kernels (P < 0.05). ST, sterigmatocystin standard. dpi, days post infection.