Seasonal variation and wing development in Junonia coenia. (a) Different light and temperature conditions can induce North Carolina J. coenia to display extremely different seasonal morphs. (b) The four developmental stages sampled in this study: (1) late 5th instar hindwing discs, (2) preommochrome pupal hind-wings, (3) early ommochrome pupal hindwings, and (4) late ommochrome pupal hindwings. (c) Comparisons of transfrag similarity between Heliconius melpomene, Danaus plexippus and J. coenia using OrthoMCL from amino acid sequences. Note that the complete genomes have been sequenced for H. melpomene and D. plexippus, while J. coenia is represented only by wing tissue-specific transcripts. (d) A unified heat map illustrating the differential expression of 3145 loci between four developmental stages and two colour morphs of J. coenia. The diagram shows the relative expression levels from all samples compared against background levels. The relative levels of upregulation or downregulation are represented by a coloured field that ranges from yellow for highly upregulated genes to blue for highly downregulated genes.