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Zuc-dependent phasing of primary piRNAs. (A) Ping-Pong analysis of all piRNAs from w1, aubHN2/QC42; ago3t2/t3 and zucHM27/Df. (B) Distance from the 3′ ends of upstream piRNAs to the 5′ ends of downstream piRNAs on the same genomic strand. (C) Nucleotide composition immediately after the 3′ ends of uniquely mapping 42AB piRNAs. Z10: Ping-Pong; Z1: phasing.
