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Contribution of maternal and secondary piRNAs to phasing. (A) Ping-Pong, phasing, and +1U percentage were analyzed for P{GSV6}42A18-derived piRNAs inherited paternally or maternally, with or without Vasa. (B) Length distribution of Piwi-bound, uniquely mapping P{GSV6}42A18 piRNAs. Reads were normalized to flamenco-derived, uniquely mapping piRNAs.
