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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Endocr Relat Cancer. 2015 Jul 10;22(5):713–724. doi: 10.1530/ERC-15-0287

Fig. 3. The in vivo pharmacology of RAD1901 is influenced by SERD activity.

Fig. 3

Ovariectomized C57Bl/6 mice (n = 5) were treated daily for 3 days with (A) vehicle or (B) estradiol (10 μg/kg) together with vehicle, Ralox (10 mg/kg) or RAD (0.3–100 mg/kg). At euthanasia, body weight as well as uterine wet weight were measured prior to cryopreservation of the uterus. Significant changes in uterine weight observed in animals not receiving estrogen treatment (A) were determined by ANOVA followed by Bonferroni comparison to the vehicle control and are indicated (* p < 0.05). C) ESR1 and β-actin expression in extracts made from pulverized uterine tissues were analyzed by immunoblot as in Fig. 1 (left). ESR1 expression relative to β-actin was quantitated as in Fig. 2 (right). Significant downregulation (* p < 0.05) of ESR1 was determined by ANOVA followed by Bonferroni multiple comparison.