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. Author manuscript; available in PMC: 2016 Aug 18.
Published in final edited form as: Cell Rep. 2015 Aug 6;12(7):1107–1119. doi: 10.1016/j.celrep.2015.07.015

Figure 2. Macrophages are associated with regions of the seminiferous tubule containing ZBTB16-expressing undifferentiated spermatogonia.

Figure 2

Immunofluorescence images of adult cryosectioned testis (A) and whole-mount seminiferous tubules (B, C). A’ is a higher magnification of the boxed region in A. Macrophages (A, arrowheads) were located adjacent to ZBTB16-expressing cells and extended long cellular processes (A’) over ZBTB16-positive cells. Tubule regions populated by fewer macrophages (B) had fewer ZBTB16-positive cells, as compared to high-density macrophage regions (C). Thin scale bar, 50 µm; thick scale bar, 25 µm. (D) Counts of macrophages per unit area for tubule regions of various stages of the spermatogenic cycle. *, P<0.01, **, P<0.001. (E) Counts of ZBTB16-bright and ZBTB16-dim cells at various stages of the spermatogenic cycle. Data are represented as mean ± SEM. Cartoon depicts stages of the spermatogenic cycle and representative numbers of ZBTB16-bright and ZBTB16-dim spermatogonia relative to macrophages along the tubule surface. See also Figure S3.