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. Author manuscript; available in PMC: 2016 Aug 18.
Published in final edited form as: Cell Rep. 2015 Aug 6;12(7):1107–1119. doi: 10.1016/j.celrep.2015.07.015

Figure 7. The expression of CSF1 and RA synthesis enzymes are dysregulated in macrophage-depleted adult testes.

Figure 7

Immunofluorescent images of adult DT-injected Cre-negative Rosa-iDTR control (A, C, E) and DT-injected Cx3cr1-Cre; Rosa-iDTR macrophage-depleted (B, D, F) testes. Black-and-white images are CSF1-only (A, B), ALDH1A2-only (C, D), and RDH10-only (E, F) channels of the panel immediately to its left. In macrophage-depleted samples, CSF1 expression was no longer enriched in perivascular regions (A, B, asterisk), and ALDH1A2 (C, D) and RDH10 (E, F) expression were reduced. Arrow in E denotes a MHCII-positive, RDH10-expressing peritubular macrophage; arrowheads in E and F point to RDH10-negative peritubular myoid cells. All images are from cryosectioned testes. Scale bar, 50 µm.