Immunofluorescent images of adult DT-injected Cre-negative Rosa-iDTR control (A, C, E) and DT-injected Cx3cr1-Cre; Rosa-iDTR macrophage-depleted (B, D, F) testes. Black-and-white images are CSF1-only (A, B), ALDH1A2-only (C, D), and RDH10-only (E, F) channels of the panel immediately to its left. In macrophage-depleted samples, CSF1 expression was no longer enriched in perivascular regions (A, B, asterisk), and ALDH1A2 (C, D) and RDH10 (E, F) expression were reduced. Arrow in E denotes a MHCII-positive, RDH10-expressing peritubular macrophage; arrowheads in E and F point to RDH10-negative peritubular myoid cells. All images are from cryosectioned testes. Scale bar, 50 µm.