Fig. 3.

Sal-YB1 could upregulate TLR4 expression and downregulate IRAK and IκBα in tumor tissues. a Flow cytometry of TLR4. Dashed line, gray filled histogram, continuous line represented isotype control, YB1-untreated and -treated mice, respectively. Based on the isotype control, TLR4 expression in the YB1-treated mice was gated as 50.9 %. b IRAK and IκBα Western blotting. There was a significant difference for IRAK (P < 0.05) and IκBα (P < 0.001) between the YB1-treated and -untreated groups. The band density of IRAK and IκBα were normalized to β-actin. The error bars showed means ± SEM for three nude mice in each group. *P < 0.05 and ^*** P < 0.001 by an unpaired two-tailed t test