| Day 1 | Seed cells | Well 1 | Well 2 | Well 3 |
| Day 2 | Treatment to well 3 | – | – | + |
| Day 3 | Treatment to well 2 | – | + | + |
| Day 4 | Flow (JC-1) | Control group | 1-day treatment group | 2-day treatment group |
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