(A) RT-PCR splicing analysis of Cacna1d in WT, Mbnl1ΔE3/ΔE3, Mbnl2
ΔE2/ΔE2 and Nestin-Cre DKO brain. Splicing of CACNA1D in human control, DM2, and DM1 brain shown for comparison (n = 3 per group, data are reported ± SEM, ***p < 0.001, **p < 0.01).
(B) Same as (A) but splicing analysis of mouse Grin1 compared to human GRIN1 (n = 3 per group, data are reported ± SEM, *p < 0.05).
(C) RNA splicing maps using human MBNL2 CLIP tags near exons mis-spliced in the DM1 frontal cortex (included exons, red; skipped exons, blue; coverage ≥ 20, |dI| ≥0.1, FDR ≤ 0.05). Also included is MBNL binding motif data, or YGCY motifs in the human genome near the mis-spliced exons, using a previously described computational procedure (included exons, light red; skipped exons, light blue) (Zhang et al., 2013).