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. Author manuscript; available in PMC: 2016 Aug 18.
Published in final edited form as: Cell Rep. 2015 Aug 6;12(7):1159–1168. doi: 10.1016/j.celrep.2015.07.029

Figure 4. DM-relevant mis-splicing in Nestin-Cre DKO brain.

Figure 4

(A) RT-PCR splicing analysis of Cacna1d in WT, Mbnl1ΔE3/ΔE3, Mbnl2 ΔE2/ΔE2 and Nestin-Cre DKO brain. Splicing of CACNA1D in human control, DM2, and DM1 brain shown for comparison (n = 3 per group, data are reported ± SEM, ***p < 0.001, **p < 0.01).

(B) Same as (A) but splicing analysis of mouse Grin1 compared to human GRIN1 (n = 3 per group, data are reported ± SEM, *p < 0.05).

(C) RNA splicing maps using human MBNL2 CLIP tags near exons mis-spliced in the DM1 frontal cortex (included exons, red; skipped exons, blue; coverage ≥ 20, |dI| ≥0.1, FDR ≤ 0.05). Also included is MBNL binding motif data, or YGCY motifs in the human genome near the mis-spliced exons, using a previously described computational procedure (included exons, light red; skipped exons, light blue) (Zhang et al., 2013).