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Primers for qPCR
| Marker | Forward primer Backward primer | Primer location within CDS | Target size | NCBI Accession |
|---|---|---|---|---|
| IL-12p35 | AAGGCCAGACAAACTCTAGAATTC | Exon 3/4 and 4/5 from ∼8 | 116 nts | XM_002716291 |
| TTGGTTAACTCCAGTGGTAAACAGG | ||||
| iNOS | GACGTCCAGCGCTACAATATCC | Undetermined | 102 nts | XM_002718780 |
| GATCTCTGTGACGGCCTGATCT | ||||
| IFNγ | TGCCAGGACACACTAACCAGAG | Exon 1 and 2/3 from 4 | 127 nts | NM_001081991 |
| TGTCACTCTCCTCTTTCCAATTCC | ||||
| GAPDH | TGACGACATCAAGAAGGTGGTG | Exon 1 of 1 | 120 nts | NM_001082253 |
| GAAGGTGGAGGAGTGGGTGTC |
