Table 1.
Protein array densitometry of Matrigel
| Array no. | Angiogenesis array | Mean | SD |
|---|---|---|---|
| A1, A2, A21, A22, F1, F2 | Positive control | 100.00 | 17.50 |
| F19, F20 | Negative control | 1.01 | 1.02 |
| A5, A6 | ADAMTS1 | 0.39 | 0.57 |
| A7, A8 | Amphiregulin | 0.12 | 0.54 |
| A9, A10 | Angiogenin | 6.51 | 2.06 |
| A11, A12 | Angiopoietin-1 | 0.80 | 0.43 |
| A13, A14 | Angiopoietin-3 | 0.30 | 0.51 |
| A15, A16 | Coagulation factor III | 36.60 | 5.60 |
| A17, A18 | CXCL16 | 18.31 | 7.42 |
| B3, B4 | Cyr61 | 22.18 | 8.34 |
| B5, B6 | DLL4 | 0.69 | 0.26 |
| B7, B8 | DPPIV | 1.76 | 1.07 |
| B9, B10 | EGFa | 0.41 | 0.46 |
| B11, B12 | Endoglin/CD105 | 22.07 | 3.44 |
| B13, B14 | Endostatin/Collagen XVIII | 38.36 | 6.22 |
| B15, B16 | Endothelin-1 | 29.04 | 6.73 |
| B17, B18 | FGF-1/aFGF | 5.45 | 2.28 |
| B19, B20 | FGF-2/bFGFa | 1.49 | 0.79 |
| C3, C4 | FGF-7/KGF | 0.65 | 0.49 |
| C5, C6 | Fractalkine/CX3CL1 | 1.11 | 0.39 |
| C7, C8 | GM-CSF | 0.72 | 0.83 |
| C9, C10 | HB-EGF | 1.65 | 0.35 |
| C11, C12 | HGF | 2.62 | 1.15 |
| C13, C14 | IGFBP-1 | 125.98 | 21.66 |
| C15, C16 | IGFBP-2 | 62.86 | 11.38 |
| C17, C18 | IGFBP-3 | 231.04 | 33.77 |
| C19, C20 | IL-1α | 2.92 | 1.16 |
| C21, C22 | IL-1β | 1.18 | 1.04 |
| D3, D4 | IL-10 | 1.03 | 0.26 |
| D5, D6 | IP-10/CXCL10 | 3.70 | 1.25 |
| D7, D8 | KC/CXCL1/GROα | 0.85 | 0.29 |
| D9, D10 | Leptin | 0.89 | 0.31 |
| D11, D12 | MCP-1/CCL2/JE | 44.18 | 17.68 |
| D13, D14 | MIP-1α/CCL3 | 12.02 | 4.06 |
| D15, D16 | MMP-3 (pro/mature form) | 100.87 | 35.72 |
| D17, D18 | MMP-8 (pro form) | 10.61 | 3.68 |
| D19, D20 | MMP-9 (pro/active form) | 36.99 | 6.05 |
| D21, D22 | NOV/CCN3 | 57.01 | 14.70 |
| E3, E4 | Osteopontin | 33.68 | 4.38 |
| E5, E6 | PD-ECGF | 1.65 | 0.44 |
| E7, E8 | PDGF-AA | 1.90 | 0.26 |
| E9, E10 | PDGF-AB/PDGF-BBa | 1.32 | 0.37 |
| E11, E12 | Pentraxin-3/TSG-14 | 23.18 | 6.39 |
| E13, E14 | Platelet factor-4/CXCL4 | 123.71 | 20.97 |
| E15, E16 | PlGF-2 | 112.56 | 30.26 |
| E17, E18 | Prolactin | 2.79 | 1.33 |
| E19, E20 | Proliferin | 1.85 | 1.26 |
| F3, F4 | SDF-1/CXCL12 | 3.34 | 0.92 |
| F5, F6 | Serpin E1/PAI-1 | 160.49 | 8.97 |
| F7, F8 | Serpin F1/PEDFb | 12.37 | 2.05 |
| F9, F10 | Thrombospondin-2 | 1.28 | 0.52 |
| F11, F12 | TIMP-1 | 27.11 | 4.28 |
| F13, F14 | TIMP-4 | 9.23 | 3.17 |
| F15, F16 | VEGF | 142.52 | 29.32 |
| F17, F18 | VEGF-B | 4.67 | 1.09 |
| Array no. | Cytokine array | Mean | SD |
|---|---|---|---|
| A1, A2, A23, A24, F1, F2 | Positive control | 100.01 | 17.32 |
| F23, F24 | PBS (negative control) | 1.39 | 1.24 |
| B1, B2 | BLC/CXCL13 | 2.05 | 0.97 |
| B3, B4 | C5a | 127.99 | 16.76 |
| B5, B6 | G-CSF | 1.75 | 0.52 |
| B7, B8 | GM-CSF | 0.56 | 0.40 |
| B9, B10 | I-309/CCL1 | 0.67 | 0.48 |
| B11, B12 | Eotaxin/CCL11 | 0.48 | 0.65 |
| B13, B14 | sICAM-1/CD54 | 96.60 | 19.23 |
| B15, B16 | IFN-γ | 1.43 | 0.20 |
| B17, B18 | IL-1α | 1.81 | 0.87 |
| B19, B20 | IL-1β | 1.96 | 1.43 |
| B21, B22 | IL-1ra | 43.89 | 23.18 |
| B23, B24 | IL-2 | 1.57 | 1.11 |
| C1, C2 | IL-3 | 1.52 | 0.95 |
| C3, C4 | IL-4 | 3.37 | 1.31 |
| C5, C6 | IL-5 | 0.55 | 0.12 |
| C7, C8 | IL-6 | 0.56 | 0.39 |
| C9, C10 | IL-7 | 2.12 | 0.75 |
| C11, C12 | IL-10 | 1.10 | 0.77 |
| C13, C14 | IL-13 | 5.60 | 2.85 |
| C15, C16 | IL-12 p70 | 1.21 | 0.65 |
| C17, C18 | IL-16 | 7.14 | 6.71 |
| C19, C20 | IL-17 | 1.56 | 1.25 |
| C21, C22 | IL-23 | 4.92 | 3.17 |
| C23, C24 | IL-27 | 1.52 | 1.50 |
| D1, D2 | IP-10/CXCL10 | 22.86 | 13.55 |
| D3, D4 | I-TAC/CXCL11 | 7.06 | 1.83 |
| D5, D6 | KC/CXCL1/GROα | 1.92 | 0.52 |
| D7, D8 | M-CSF/CSF-1 | 4.39 | 1.42 |
| D9, D10 | MCP-1/CCL2/JE | 95.71 | 18.61 |
| D11, D12 | MCP-5/CCL12 | 20.07 | 2.49 |
| D13, D14 | MIG/CXCL9 | 145.30 | 39.04 |
| D15, D16 | MIP-1α/CCL3 | 4.73 | 1.90 |
| D17, D18 | MIP-1β/CCL4 | 1.64 | 1.09 |
| D19, D20 | MIP-2/CXCL2 | 2.09 | 1.51 |
| D21, D22 | RANTES/CCL5 | 89.25 | 52.54 |
| D23, D24 | SDF-1/CXCL12 | 2.21 | 1.37 |
| E1, E2 | TARC/CCL17 | 3.98 | 1.59 |
| E3, E4 | TIMP-1 | 241.36 | 27.62 |
| E5, E6 | TNF-α | 2.34 | 0.54 |
| E7, E8 | TREM-1 | 0.92 | 0.72 |
| Array no. | Adipokine array | Mean | SD |
|---|---|---|---|
| A1, A2, A23, A24, F1, F2 | Positive control | 100.00 | 17.58 |
| F23, F24 | PBS (negative control) | 1.65 | 3.17 |
| B1, B2 | Adiponectin | 92.55 | 6.89 |
| B3, B4 | AgRP | 1.72 | 0.83 |
| B5, B6 | ANGPT-L3 | 1.89 | 0.61 |
| B7, B8 | C-reactive protein | 43.67 | 10.55 |
| B9, B10 | DPPIV | 2.88 | 1.67 |
| B11, B12 | Endocan/EMS-1 | 106.74 | 31.06 |
| B13, B14 | Fetuin A | 19.82 | 7.28 |
| B15, B16 | FGF acidic | 0.73 | 0.56 |
| B17, B18 | FGF-21 | 0.05 | 0.13 |
| B19, B20 | HGF | 0.30 | 0.20 |
| B21, B22 | ICAM-1 | 44.00 | 8.32 |
| B23, B24 | IGF-1a | 2.33 | 1.39 |
| C1, C2 | IGF-2 | 3.48 | 1.55 |
| C3, C4 | IGFBP-1 | 72.15 | 8.38 |
| C5, C6 | IGFBP-2 | 40.94 | 6.24 |
| C7, C8 | IGFBP-3 | 134.18 | 11.20 |
| C9, C10 | IGFBP-5 | 4.87 | 3.32 |
| C11, C12 | IGFBP-6 | 47.42 | 19.39 |
| C13, C14 | IL-6 | 1.18 | 0.92 |
| C15, C16 | IL-10 | 1.48 | 0.78 |
| C17, C18 | IL-11 | 0.76 | 0.48 |
| C19, C20 | Leptin | 1.95 | 1.00 |
| C21, C22 | LIF | 94.83 | 16.67 |
| C23, C24 | Lipocalin-2 | 142.33 | 8.73 |
| D1, D2 | MCP-1/CCL2/JE | 30.51 | 11.74 |
| D3, D4 | M-CSF//CSF-1 | 18.73 | 3.69 |
| D5, D6 | Oncostatin M | 1.72 | 0.80 |
| D7, D8 | Pentraxin-2 | 7.35 | 3.27 |
| D9, D10 | Pentraxin-3/TSG-14 | 16.33 | 10.58 |
| D11, D12 | Pref-1/DLK-1 | 1.13 | 0.66 |
| D13, D14 | RAGE | 0.63 | 0.45 |
| D15, D16 | RANTES/CCL5 | 46.70 | 22.04 |
| D17, D18 | RBP4 | 17.01 | 5.61 |
| D19, D20 | Resistin | 63.66 | 15.75 |
| D21, D22 | Serpin E1/PAI-1 | 91.93 | 13.82 |
| D23, D24 | TIMP-1 | 119.32 | 16.48 |
| E1, E2 | TNF-α | 2.66 | 0.94 |
| E3, E4 | VEGF | 124.05 | 10.27 |
aProteins previously reported in Matrigel as detected by immunoassay
bProteins previously reported in Matrigel as detected by mass spectroscopy