Table 1.
Mitochondrial outcomes in lymphocytes from patient and controls.
| Outcome | Patient | Mother | Father |
|---|---|---|---|
| NNT activity/NNT protein levels (× 1000) | 0.57 ± 0.02 | 1.00 ± 0.003 | 1.04 ± 0.03 |
| Range | [1.27, 2.40] | ||
| Nitrotyrosine (normalized by GAPDH) | 10.1 ± 2.9 | 7.7 ± 3.0 | 6.2 ± 0.4 |
| Range | [3.4, 5.6] | [4.3, 10.6] | |
| Citrate synthase activity | 2.1 ± 0.1 | 1.7 ± 0.1 | 4.6 ± 0.8 |
| 95%CI | [6,12] | ||
| CCO/CS activity | 1.8 ± 0.4 | 4.2 ± 0.5 | 2.3 ± 0.3 |
| Range | [2.1, 4.4] | ||
Bold numbers indicate values outside either the 95%CI (calculated with control values) or Range (when the number of control individuals for a particular outcome was < 4, the range represents the lowest and highest values obtained for that outcome). When no statistically significant difference was observed between younger and older control individuals only one combined 95%CI or range was reported instead of two. The 95%CI for citrate synthase activity was calculated using data from healthy individuals aged 20 to 30 y (n = 38) and 40 to 60 y (n = 12) that matched the age of the parents. NNT activity, CCO activity and CS activity were all expressed as nmol × (min × 106 cells)− 1. NNT activity was then normalized to the intensity of the NNT band obtained by Western blots to express the specific activity. AUD, arbitrary units of density of the nitrated protein band normalized to an unknown protein used as loading control.