FIG. 8.

Postnatal PCFUs with tri-lineage potential can be enriched by CD133^high expression. (A) Representative flow cytometry analysis of CD133 expression on postnatal pancreatic cells. Three distinct populations were observed: CD133^high, CD133^low, and CD133^negative. (B) qRT-PCR analysis of CD133 and Mucin1 genes in freshly sorted CD133^high, CD133^low, CD133^negative cells, and unsorted pancreatic cells from postnatal mice. (C) Cyst colony-forming efficiency in Matrigel-supplemented culture from CD133^high, CD133^low, and CD133^negative cells, expressed as fold change over unsorted cells. (D) Schematic of single cell micromanipulation experiment. (E) Representative images of colonies derived from a single CD133^high cell, grown over 17 days in cultures containing 5% Matrigel. (F) Microfluidic qRT-PCR analysis of five individual colonies formed from single CD133^high cells, collected after 17 days in culture.