Fig 5. Immunolocalization of Ts-ES-1 in sections of T. spiralis muscle larvae (400×).

Fluorescence was detected with Dylight 488-conjugated anti-mouse IgG secondary antibody. Longitudinal sections of T. spiralis ML were probed with mouse anti-Ts-ES-1 antisera (1:100) and native Ts-ES-1 was shown to be located intensively in the stichocytes at the posterior portion of T. spiralis ML stichosome (A). Nothing was detected when normal mouse serum at the same dilution was used as control (B).