Figure 1.
Antibody profiling on HD-viral-NAPPA. A. Step 1: Production of HD-viral-NAPPA by printing plasmid DNA and capture antibody into Silicon NanoWells (SiNWs); Step 2: Protein display on HD-viral-NAPPA after isolated expression and in situ capture in individual wells; Step 3: Antibody profiling to detect distinct sero-reactivity against individual antigens; B. Images of a SiNW substrate by scanning electron microscopy (top), proteins displayed on HD-viral-NAPPA as detected by anti-GST antibody (middle), and sero-profiling on HD-viral-NAPPA (bottom).