. Author manuscript; available in PMC: 2015 Aug 22.

Published in final edited form as: J Nucl Med. 2014 Nov 7;55(12):2057–2063. doi: 10.2967/jnumed.114.145896

Table 3.

Biodistribution of ^99mTc(EDDA)-HYNIC-AocNle-CycMSH_hex in B16/F1 melanoma-bearing C57 mice. The data were presented as percent injected dose/gram or as percent injected dose (Mean ± SD, n=5)

Tissues	0.5 h	2 h^#	4 h	24 h	2-h NDP blockade	2-h L-Lys co-injection
	Percent injected dose/gram (%ID/g)
Tumor	23.44 ± 3.37	22.8 ± 1.71	22.17 ± 5.93	7.13 ± 0.99	1.26 ± 0.59^*	25.85 ± 6.8
Brain	0.17 ± 0.07	0.05 ± 0.03	0.02 ± 0.02	0.01 ± 0.01	0.03 ± 0.01	0.03 ± 0.01
Blood	2.98 ± 1.77	0.40 ± 0.14	0.30 ± 0.14	0.09 ± 0.03	0.82 ± 0.15	0.18 ± 0.13
Heart	1.75 ± 0.31	0.23 ± 0.08	0.13 ± 0.09	0.07 ± 0.02	0.32 ± 0.09	0.14 ± 0.01
Lung	4.06 ± 1.60	0.52 ± 0.20	0.50 ± 0.19	0.14 ± 0.02	0.91 ± 0.06	0.59 ± 0.06
Liver	3.19 ± 0.39	1.09 ± 0.33	1.26 ± 0.60	0.36 ± 0.01	1.22 ± 0.12	1.22 ± 0.15
Spleen	0.94 ± 0.72	0.30 ± 0.13	0.32 ± 0.13	0.05 ± 0.04	0.17 ± 0.11	0.22 ± 0.09
Stomach	2.52 ± 0.22	1.07 ± 0.10	0.73 ± 0.21	0.11 ± 0.02	0.56 ± 0.10	0.97 ± 0.36
Kidneys	19.65 ± 7.36	6.92 ± 1.40	6.10 ± 0.72	1.05 ± 0.07	6.05 ± 1.13	4.64 ± 0.48^*
Muscle	0.43 ± 0.14	0.04 ± 0.03	0.04 ± 0.03	0.02 ± 0.01	0.06 ± 0.05	0.07 ± 0.06
Pancreas	0.97 ± 0.40	0.19 ± 0.14	0.44 ± 0.64	0.06 ± 0.03	0.31 ± 0.10	0.06 ± 0.02
Bone	1.09 ± 0.28	0.15 ± 0.09	0.19 ± 0.15	0.12 ± 0.07	0.30 ± 0.08	0.33 ± 0.30
Skin	3.62 ± 0.35	0.46 ± 0.10	0.37 ± 0.11	0.15 ± 0.05	0.45 ± 0.06	0.54 ± 0.10

	Percent injected dose (%ID)
Intestines	2.43 ± 0.31	1.85 ± 0.51	3.43 ± 0.76	0.32 ± 0.09	1.85 ± 0.42	1.64 ± 0.40
Urine	60.22 ± 4.46	88.46 ± 2.23	84.47 ± 4.80	96.84 ± 1.17	91.29 ± 0.70	88.11 ± 1.90

	Uptake ratio of tumor/normal tissue
Tumor/liver	7.34	20.86	17.62	20.05	1.03	21.19
Tumor/kidney	1.19	3.29	3.63	6.78	0.21	5.57
Tumor/lung	5.78	43.77	44.51	52.05	1.39	44.16
Tumor/muscle	54.62	527.80	502.79	450.68	21.12	392.26
Tumor/blood	7.87	57.59	74.61	82.89	1.54	139.85
Tumor/skin	6.47	49.13	59.92	47.99	2.82	47.44

2 h Data were cited from Table 2 for comparison;

p<0.05 for determining significance of differences in tumor and kidney uptake between ^99mTc(EDDA)-HYNIC-AocNle-CycMSH_hex with or without peptide blockade at 2 h post-injection.