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. Author manuscript; available in PMC: 2015 Aug 22.
Published in final edited form as: Stem Cells. 2012 Nov;30(11):2366–2377. doi: 10.1002/stem.1219

Figure 6. HDAC inhibitors reduce proliferation and the percentage of cycling cells.

Figure 6

Cell proliferation assay conducted to assess proliferation rates in (A) ALDH− or (B) ALDH+ cell population pre-treated for 7 days with 1mM VA or 1μM SAHA or vehicle control. (C) Assessment of apoptosis in ALDH− and ALDH+ P0 SUM159 cells pretreated for 7 days with 1mM VA, 1μM SAHA or vehicle control. The induction of apoptosis was determined by flow cytometric analysis of Annexin V-FITC and PI-staining. Cells in the lower right quadrant indicate Annexin-positive, early apoptotic cells while cells in the upper right quadrant indicate Annexin-positive/PI-positive, late apoptotic cells. (D) ALDH− and (E) ALDH+ SUM159 cells of multiple passages were profiled for their cell cycle pattern after they were pretreated with 1mM VA, 1μM SAHA or vehicle for 7 days. (F) Western blot analysis showed increased levels of cell cycle inhibitors p21 and p27 in VA-treated ALDH− P0 cells.