Table I.
Primary Antibodies Used
Antigen | Description of Immunogen | Source, host species, catalog No., clone or lot No., RRID | Concentration used (μg/ml) | Primary antibody blocking solution |
---|---|---|---|---|
β-Actin | C-terminal actin fragment: Ser-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe | Sigma-Aldrich (St. Louis, MO), rabbit polyclonal, A2066, AB_476693 | 0.2 | 3% Nonfat rrilk-PBS 0.1% Tween-20 |
pERK1/2 | A synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase | Cell Signaling Technology (Beverly, MA), rabbit polyclonal, 9101S, lot No. 28, AB_331646 | 1 | 3% Nonfat milk-PBS 0.1% Tween-20 |
ERK1/2 | A synthetic peptide corresponding to a sequence in the C-terminus of rat p44 MAP Kinase | Cell Signaling Technology, rabbit polyclonal, 9102S, lot No. 23, AB_10695746 | 0.5 | 3% Nonfat milk-PBS 0.1% Tween-20 |
pJNK | A synthetic phosphopeptide corresponding to residues surrounding Thr183/Tyr185 of human SAPK/JNK. | Cell Signaling Technology, rabbit polyclonal, 9251L, lot No. 21, AB_2140557 | 1 | 3% Nonfat milk-PBS 0.1% Tween-20 |
JNK | A recombinant human JNK2 protein | Cell Signaling Technology, rabbit polyclonal, 9252S, lot No. 12, AB_10693936 | 0.5 | 3% Nonfat milk-PBS 0.1% Tween-20 |