Fig. 5.

Effect of Detergent on Suramin Inhibition. a NTS IC₅₀ measurement in the presence and absence of 0.5 % Triton, showing no detergent-effect on inhibition of DUSP5 PD(WT) pNPP phosphatase activity. b Suramin IC₅₀ measurement in the presence and absence of 0.5 % Triton X-100 shows a loss of some inhibitory capability in the presence of detergent. c Effect of increasing detergent levels (Triton X-100) on rate of DUSP5 PD(WT) in the presence of a fixed concentrations of inhibitor and substrate (at 1 μM suramin and 5 mM pNPP). Detergent removes some, but not all, of suramin’s inhibitory effect, showing a plateau level 30 % inhibition