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. 2015 Aug 19;10(8):e0136066. doi: 10.1371/journal.pone.0136066

Table 1. Primer sequences for semi-quantitative RT-PCR analysis.

mRNA Oligonucleotide
GAPDH Forward 5’- GGGCTGCTTTTAACTCTGGT-3’
Reverse 5’- TGGCAGGTTTTTCTAGACGG-3’
ITGA5 Forward 5’- GCAAGAGCCGGATAGAGGAC-3’
Reverse 5’-GGACTGTAAACCGAAGGCCA-3’
ITGB3 Forward 5’-CTGCCGTGACGAGATTGAGT-3’
Reverse 5’-ATTAAGTGCCCCGGTACGTG-3’

GAPDH was designed according to Tuli et al. [30]. ITGA5 and ITGB3 were designed in our laboratory.